14 May 2018 Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources
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Stimulated Raman scattering microscopy allows vibrational contrast mechanism for imaging with high spectral and spatial resolution along with three-dimensional sectioning. In this paper, the implementation of a Stimulated Raman Scattering microscope (SRSM), obtained by the integration of a femtosecond SRS spectroscopy set-up with an optical microscope equipped with a scanning unit, is described. Femtosecond Stimulated Raman Scattering microscope is equipped with three femtosecond laser sources: a Ti:Sapphire (Ti:Sa), a synchronized optical parametric oscillator (SOPO) and a frequency converters for ultrafast lasers, i.e. a second harmonic generator optimized for the SOPO. The proposed implementation allows to cover all the regions of Raman spectra, taking advantage of two different laser combinations. The first, Ti:Sa and SHG laser combination can cover in SRL modality the fingerprint region (500 − 1700 cm−1 ) and the silent region. The second, Ti:Sa and OPO, can cover the C-H region or O−H region (2800 − 3200 cm−1 ) in SRG modality. In order to demonstrate its successful realization Stimulated Raman Gain (SRG) and Stimulated Raman Losses (SRL) images of polystyrene beads are reported and discussed.
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Rajeev Ranjan, Rajeev Ranjan, Annalisa D'Arco, Annalisa D'Arco, Maria Antonietta Ferrara, Maria Antonietta Ferrara, Maurizio Indolfi, Maurizio Indolfi, Michele Larobina, Michele Larobina, Luigi Sirleto, Luigi Sirleto, } "Implementation of stimulated Raman losses and stimulated Raman gain microscopy using three femtosecond laser sources", Proc. SPIE 10684, Nonlinear Optics and its Applications 2018, 106842J (14 May 2018); doi: 10.1117/12.2315967; https://doi.org/10.1117/12.2315967

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