Combined label-free/fluorescence platform based on Bloch surface waves biochips for cancer biomarker detection

Norbert Danz; Alberto Sinibaldi; Matteo Allegretti; Elisabetta Sepe; Peter Munzert; Patrizio Giacomini; Francesco Michelotti

doi:10.1117/12.2306424

17 May 2018 Combined label-free/fluorescence platform based on Bloch surface waves biochips for cancer biomarker detection

Norbert Danz, Alberto Sinibaldi, Matteo Allegretti, Elisabetta Sepe, Peter Munzert, Patrizio Giacomini, Francesco Michelotti

Author Affiliations +

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106850X (2018) https://doi.org/10.1117/12.2306424
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

A biosensor platform based on Bloch Surface Waves and operating in angular interrogation mode is applied to the detection of a clinical biomarker (HER2-neu/ERBB2) related to breast cancer initiation/progression. Preparing regions for specific recognition of different proteins as well as a reference on the biochip enables to correct the signal for nonspecific effects. Additionally, label-free analysis and surface wave enhanced fluorescence detection can be applied and compared directly on the platform. Cell lysates with high and low expression levels of ERBB2 are analyzed. Comparing the signals of such ERBB2 positive and negative samples estimates the limit of detection at 1.7 ng/mL. This is well below the threshold of 15 ng/mL set by the FDA for clinically useful ERBB2 detection in human serum, demonstrating that 1DPC-based biochips are attractive candidates for breast cancer detection/monitoring.

Citation Download Citation

Norbert Danz, Alberto Sinibaldi, Matteo Allegretti, Elisabetta Sepe, Peter Munzert, Patrizio Giacomini, and Francesco Michelotti "Combined label-free/fluorescence platform based on Bloch surface waves biochips for cancer biomarker detection", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106850X (17 May 2018); https://doi.org/10.1117/12.2306424

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