17 May 2018 Measurement of skin autofluorescence by fluorescent spectrometry for diabetes diagnostics and control
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Abstract
Introduction. Fluorescence spectrometry allows studying skin autofluorescence (AF), which shows content of advanced glycation end products. The aim of study was to determine possibility of using the experimental sample of fiber optic spectrometer FOS-1 for diabetes mellitus diagnostics and control, including noninvasive diagnostics of complications.

Methods. We involved 36 healthy participants, 13 type 1 diabetic patients, 10 type 2 diabetic patients. The 1st and the 2nd groups were comparable by the age, gender, skin reflection coefficient, characterizing skin phototype and degree of tanning. Skin AF was measured at a wavelength of 460 nm with excitation of 365 nm. To reduce effect of skin pigmentation, ratio of fluorescent signal to signal of reflection in excitation region was used as measured parameter.

Results. Significant correlation between AF intensity and age was found in type 1 diabetic and control groups (R=0.6, р<0.05 and R=0.43, p<0.05, respectively). No significant difference in AF level was found between these groups: median AF was 0.87 (0.86; 0.89) arb. units and 0.85 (0.77; 0.88) arb. units respectively. In type 1 diabetic group AF also positively correlated, although not statistically significantly, with diabetes duration, glycosylated hemoglobin level, average daily glucose level (R=0.52, p=0.06; R=0.45, p=0.09 and R=0.56, p=0.07 respectively). The median AF was 14.7% higher (p=0.34) in patients with several diabetes complications than in diabetics with 1 complication and 13.9% (р=0.19) higher than in patients without complications.

Conclusion. Obtained data show possibility of using the described method with spectrometer FOS-1 for diabetes control and for diagnostics of microvascular complications.
Conference Presentation
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Yulia A. Kononova, Anastasiya G. Arkhipova, Garry V. Papayan, Mikhail K. Khodzitsky, Alina Yu. Babenko, Elena N. Grineva, "Measurement of skin autofluorescence by fluorescent spectrometry for diabetes diagnostics and control ", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106852N (17 May 2018); doi: 10.1117/12.2307250; https://doi.org/10.1117/12.2307250
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