Vibrational spectroscopy of tissue-engineered structures based on proteins, chitosan, and carbon nanotube conjugates

A. A. Polokhin; Yu. O. Fedorova; A. Yu Gerasimenko

doi:10.1117/12.2306639

17 May 2018 Vibrational spectroscopy of tissue-engineered structures based on proteins, chitosan, and carbon nanotube conjugates

A. A. Polokhin, Yu. O. Fedorova, A. Yu Gerasimenko

Author Affiliations +

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106853H (2018) https://doi.org/10.1117/12.2306639
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

In this work, tissue-engineered structures based on a matrix of protein conjugates, chitosan and carbon nanotubes were prepared and studied. Bovine serum albumin (BSA), bovine collagen (BС) were used. Two types of single-walled carbon nanotubes (SWCNTs) were used to form a strong internal scaffold in a protein-chitosan matrix under the action of laser radiation. Tissue-engineered structures were created by means of layered deposition and laser evaporation of the initial aqueous dispersion from SWCNT, BSA, BC and chitosan succinate. As sources of laser radiation, a continuous diode laser with a wavelength of 810 nm and a pulsed fiber laser with a wavelength of 1064 nm and frequency of 80 kHz were used. Studies of tissue-engineered structures were carried out using vibrational spectroscopy methods (IR and Raman). The changes in the frequencies and intensities of the corresponding absorption bands and Raman lines of the amide group oscillations were analyzed. IR spectra of tissue-engineered structures demonstrated a high degree of binding of organic (protein, chitosan) and inorganic (SWCNT) components. The structure and defectiveness of the carbon nanotube scaffold were investigated in the Raman spectra.

Citation Download Citation

A. A. Polokhin, Yu. O. Fedorova, and A. Yu Gerasimenko "Vibrational spectroscopy of tissue-engineered structures based on proteins, chitosan, and carbon nanotube conjugates", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853H (17 May 2018); https://doi.org/10.1117/12.2306639

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