Optical monitoring of cell migration processes in a 3D scaffold

Ines Delfino; Marianna Portaccio; Antonella  D'Agostino; Marcella Cammarota; Ferdinando Campitiello; Chiara Schiraldi; Maria Lepore

doi:10.1117/12.2306982

17 May 2018 Optical monitoring of cell migration processes in a 3D scaffold

Ines Delfino; Marianna Portaccio; Antonella D'Agostino; Marcella Cammarota; Ferdinando Campitiello; Chiara Schiraldi; Maria Lepore

Author Affiliations +

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106853X (2018) https://doi.org/10.1117/12.2306982
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Three-dimensional (3D) polymeric scaffolds are utilized in tissue engineering to provide biomechanical support for the seeded cells until they are organized into a functional tissue. Studies of cell migration are important for understanding a variety of physiological and pathological processes in tissue regeneration. Optical techniques can be employed for monitoring cell migration processes in 3D scaffolds. In particular, two-photon microscopy (TPM) can be used for monitoring these processes for living cells also at considerable depths. In the present work, the cell migration process of alive chondrocytes labelled with vital PKH67 in 3D collagen-based scaffold has been monitored at different times from the seeding process. The results show that TPM can evidence the presence of living cells in different spatial regions whose localizations depend on the location and time elapsed from the seeding.

Citation Download Citation

Ines Delfino, Ines Delfino, Marianna Portaccio, Marianna Portaccio, Antonella D'Agostino, Antonella D'Agostino, Marcella Cammarota, Marcella Cammarota, Ferdinando Campitiello, Ferdinando Campitiello, Chiara Schiraldi, Chiara Schiraldi, Maria Lepore, Maria Lepore, } "Optical monitoring of cell migration processes in a 3D scaffold", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106853X (17 May 2018); doi: 10.1117/12.2306982; https://doi.org/10.1117/12.2306982

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years