Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin

Ekaterina N. Lazareva; Andrey Y. Zyubin; Ilya G. Samusev; Vasiliy A. Slezhkin; Vyacheslav I. Kochubey; Valery V. Tuchin

doi:10.1117/12.2307102

17 May 2018 Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin

Ekaterina N. Lazareva, Andrey Y. Zyubin, Ilya G. Samusev, Vasiliy A. Slezhkin, Vyacheslav I. Kochubey, Valery V. Tuchin

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 1068540 (2018) https://doi.org/10.1117/12.2307102
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

In this study, the optical properties of glycated (HbA1c) and non-glycated (Hb) hemoglobin are compared using refractometry, fluorescence and Raman spectroscopy. The fluorescence measured at an excitation wavelength of 270 nm indicates differences in the molecular structure of hemoglobins. Analysis of the spectral shift of Raman spectra also showed variations indicating differences in their molecular structure. The refractive index measured in the visible and near IR regions for different temperatures allowed for quantification of mean values of temperature increment, which are quite different as dn/dT= –(1.03 ± 0.05)×10^–4 °C^–1 for Hb and – (1.37 ± 0.07)×10^–4 °C^–1for HbA1c.The data obtained in the work can serve as a basis for further study of the optical properties of glycated hemoglobin and other glycated proteins.

Citation Download Citation

Ekaterina N. Lazareva, Andrey Y. Zyubin, Ilya G. Samusev, Vasiliy A. Slezhkin, Vyacheslav I. Kochubey, and Valery V. Tuchin "Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 1068540 (17 May 2018); https://doi.org/10.1117/12.2307102

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