Diatomite nanovectors uptake in cancer cells: a Raman imaging study

S. Managò; N. Migliaccio; M. Terracciano; M. Napolitano; N. M. Martucci; L. De Stefano; I. Rendina; A. Lamberti; I. Rea; A. C. De Luca

doi:10.1117/12.2315371

17 May 2018 Diatomite nanovectors uptake in cancer cells: a Raman imaging study

S. Managò, N. Migliaccio, M. Terracciano, M. Napolitano, N. M. Martucci, L. De Stefano, I. Rendina, A. Lamberti, I. Rea, A. C. De Luca

Author Affiliations +

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106854J (2018) https://doi.org/10.1117/12.2315371
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

In this work, we employed the Raman microscopy to study the internalization kinetics and spatial distribution of small interfering RNA (siRNA)-diatomite nanoparticles (DNPs) complex in human lung epidermoid carcinoma cell line (H1355) up to 72 h. Raman images are compared with confocal fluorescence microscopy results. The Raman analysis provides that the siRNA-DNPs are internalized and co-localized in lipid vesicles within 18 h, after that equilibrium is achieved.

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S. Managò, N. Migliaccio, M. Terracciano, M. Napolitano, N. M. Martucci, L. De Stefano, I. Rendina, A. Lamberti, I. Rea, and A. C. De Luca "Diatomite nanovectors uptake in cancer cells: a Raman imaging study", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106854J (17 May 2018); https://doi.org/10.1117/12.2315371

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KEYWORDS

Raman spectroscopy

Confocal microscopy

Microscopy

Cancer

Transmission electron microscopy

Nanoparticles

Lung cancer

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