17 May 2018 Investigating the effect of singlet oxygen on polymerase activity during modulated irradiation at photodynamic treatment
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Abstract
The goal of this study was to assess the ability of singlet oxygen generated at photodynamic treatment using different irradiation modes to inhibit the enzyme TAQ polymerase activity. Experimentally were determined the laser irradiation dose and the singlet oxygen concentration that results to complete inhibition of TAQ polymerase activity. The results show that using pulse mode irradiation is 1.5x more efficient then continuous wave. TAQ polymerase damage was also assessed by fluorescence spectrometry — tryptophan residues fluorescence is decreased if damaged by singlet oxygen during photodynamic treatment. It was observed that on doses, where TAQ polymerase looses its enzymatic activity the fluorescence decreases only by 7%. The fluorescence decrement of tryptophan correlates with damage to the enzyme.
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N. E. Kaydanov, N. E. Kaydanov, V. V. Klimenko, V. V. Klimenko, A. K. Emelyanov, A. K. Emelyanov, N. A. Knyazev, N. A. Knyazev, A. A. Bogdanov, A. A. Bogdanov, } "Investigating the effect of singlet oxygen on polymerase activity during modulated irradiation at photodynamic treatment", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106854T (17 May 2018); doi: 10.1117/12.2318952; https://doi.org/10.1117/12.2318952
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