Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spot

Takahiro Nishimura; Daiki Shinkawa; Yusuke Ogura; Yosuke Tamada; Jun Tanida

doi:10.1117/12.2319336

24 April 2018 Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spot

Takahiro Nishimura; Daiki Shinkawa; Yusuke Ogura; Yosuke Tamada; Jun Tanida

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071109 (2018) https://doi.org/10.1117/12.2319336
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

This paper presents lateral spatial resolution improvement by scanning a focused spot array pattern. To enhance the effective point spread function (PSF), we employed a phase-only computer generated hologram (CGH) that can reduce the spot size comparing to the single diffraction limited spot. A CGH was designed based on the Gerchberg-Saxton algorithm with a specific constraint to control the dispersion of light energy and the phase of generated spots. As a design example, we obtained a CGH for generating 3x3 optical spots whose sizes were reduced to 78.5% of the single diffraction limited spot. We also confirmed by simulation that the effective PSF was improved from 208 nm to 183 nm when using the subdiffraction limit spots for excitation.

Citation Download Citation

Takahiro Nishimura, Takahiro Nishimura, Daiki Shinkawa, Daiki Shinkawa, Yusuke Ogura, Yusuke Ogura, Yosuke Tamada, Yosuke Tamada, Jun Tanida, Jun Tanida, } "Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spot", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071109 (24 April 2018); doi: 10.1117/12.2319336; https://doi.org/10.1117/12.2319336

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