24 April 2018 Fast spatial domain reconstruction for structured illumination microscopy
Author Affiliations +
Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 107110N (2018) https://doi.org/10.1117/12.2309616
Event: SPIE Structured Light, 2018, Yokohama, Japan
Abstract
As a wide-field super-resolution (SR) technique, structured illumination microscopy (SIM) features the merits of fast imaging speed, low excitation intensity and a large field of view. However, it is hard for SIM to realize real-time imaging as the SR-image restoration always time-consuming. The basic workflow for conventional SIM reconstruction is based on the frequency domain reconstruction (FDR), which inevitably brings some computational errors and slows down the image reconstruction speed. In this letter, we introduce a fast SIM reconstruction scheme named spatial domain reconstruction (SDR) algorithm, which allow for obtaining a much faster reconstruction than FDR, making it suitable for real-time SR imaging. Meanwhile, the validity of the SDR algorithm is verified by real experiment.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xing Zhou, Xing Zhou, Ming Lei, Ming Lei, Dan Dan, Dan Dan, Baoli Yao, Baoli Yao, } "Fast spatial domain reconstruction for structured illumination microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 107110N (24 April 2018); doi: 10.1117/12.2309616; https://doi.org/10.1117/12.2309616
PROCEEDINGS
2 PAGES


SHARE
Back to Top