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24 April 2018 Scan-less, line-filed, confocal phase imaging with dual-comb microscopy
Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto, Takeshi Yasui
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Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071120 (2018) https://doi.org/10.1117/12.2317029
Event: SPIE Structured Light, 2018, Yokohama, Japan
Abstract
We introduce an optical frequency comb (OFC) to microscopy to coherently link the confocal microscopy and phase microscopy. One-dimensional (1D) image pixels of a sample were encoded onto OFC modes via 1D spectral encoding, in which OFC acted as an optical carrier with a vast number of discrete frequency channels. Then, a scan-less line-field confocal image with a depth resolution of 50 μm was decoded from a mode-resolved OFC amplitude spectrum obtained by dual-comb spectroscopy. Furthermore, a phase image with a depth resolution of sub-λ was decoded from a moderesolved OFC phase spectrum under the above confocality. The proposed hybrid microscopy approach will be a powerful tool for a variety of applications.
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Eiji Hase, Takeo Minamikawa, Shuji Miyamoto, Yasuhiro Mizutani, Tetsuo Iwata, Hirotsugu Yamamoto, and Takeshi Yasui "Scan-less, line-filed, confocal phase imaging with dual-comb microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071120 (24 April 2018); https://doi.org/10.1117/12.2317029
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KEYWORDS
Confocal microscopy
Microscopy
Phase imaging
Optical microscopy
Spectroscopy
Frequency combs
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