24 April 2018 Scan-less, line-filed, confocal phase imaging with dual-comb microscopy
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Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071120 (2018) https://doi.org/10.1117/12.2317029
Event: SPIE Structured Light, 2018, Yokohama, Japan
Abstract
We introduce an optical frequency comb (OFC) to microscopy to coherently link the confocal microscopy and phase microscopy. One-dimensional (1D) image pixels of a sample were encoded onto OFC modes via 1D spectral encoding, in which OFC acted as an optical carrier with a vast number of discrete frequency channels. Then, a scan-less line-field confocal image with a depth resolution of 50 μm was decoded from a mode-resolved OFC amplitude spectrum obtained by dual-comb spectroscopy. Furthermore, a phase image with a depth resolution of sub-λ was decoded from a moderesolved OFC phase spectrum under the above confocality. The proposed hybrid microscopy approach will be a powerful tool for a variety of applications.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Eiji Hase, Eiji Hase, Takeo Minamikawa, Takeo Minamikawa, Shuji Miyamoto, Shuji Miyamoto, Yasuhiro Mizutani, Yasuhiro Mizutani, Tetsuo Iwata, Tetsuo Iwata, Hirotsugu Yamamoto, Hirotsugu Yamamoto, Takeshi Yasui, Takeshi Yasui, } "Scan-less, line-filed, confocal phase imaging with dual-comb microscopy", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071120 (24 April 2018); doi: 10.1117/12.2317029; https://doi.org/10.1117/12.2317029
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