Observation of immunostained microtubules using three-dimensional super-resolution microscope with two-color annular wave plate

Yoshinori Iketaki; Nandor Bokor; Daisuke Okada; Hiroshi Kumagai

doi:10.1117/12.2319154

24 April 2018 Observation of immunostained microtubules using three-dimensional super-resolution microscope with two-color annular wave plate

Yoshinori Iketaki, Nandor Bokor, Daisuke Okada, Hiroshi Kumagai

Author Affiliations +

Proceedings Volume 10712, Optical Manipulation Conference; 1071203 (2018) https://doi.org/10.1117/12.2319154
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Three-dimensional super-resolution microscopy based on fluorescence depletion (3D-SRM) was applied to the observation of immunostained microtubules having complicated structures stacking each other. Owing to a high depth resolution overcoming the diffraction limit, we can observe the tubulin fibers intertwined in a cell with improved image quality. Since the 3D-SRM system can be setup by adopting a simple two-color annular wave plate (TAWP) to a commercial laser scanning microscope (LSM), 3D-SRM is expected to be a powerful measurement method in life science.

Citation Download Citation

Yoshinori Iketaki, Nandor Bokor, Daisuke Okada, and Hiroshi Kumagai "Observation of immunostained microtubules using three-dimensional super-resolution microscope with two-color annular wave plate", Proc. SPIE 10712, Optical Manipulation Conference, 1071203 (24 April 2018); https://doi.org/10.1117/12.2319154

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