The study of the structural features of the lymphocytes from cattle with and without retroviral infection using atomic force microscopy

Dmitry A. Artemev; Ekaterina S. Krasnikova; Olga V. Stolbovskaya; Aleksandr V. Krasnikov; Boris B. Kostishko; Roman V. Radionov

doi:10.1117/12.2317558

26 April 2018 The study of the structural features of the lymphocytes from cattle with and without retroviral infection using atomic force microscopy

Dmitry A. Artemev , Ekaterina S. Krasnikova, Olga V. Stolbovskaya, Aleksandr V. Krasnikov , Boris B. Kostishko, Roman V. Radionov

Author Affiliations +

Proceedings Volume 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX; 107160G (2018) https://doi.org/10.1117/12.2317558
Event: Saratov Fall Meeting 2017, 2017, Saratov, Russian Federation

Abstract

The results of the study of morphological and biophysical parameters of cell membranes of fixed lymphocytes from cattle with and without retroviral infection using atomic force microscopy have been presented. It is found that lymphocytes morphometric characteristics, such as diameter, height, volume, in case of BLV and BIV-BLV infection decrease by 18.5 and 22.7%, 9.0 and 25.0%, 4.4 and 35.3%, respectively, in comparison with the intact ones. It is maybe a sign of the cells immaturity or the degenerative processes development. However, the lymphocytes’ morphometric characteristics in case of BIV infection increase by 45.8%, 12.7% and 35.8%, respectively, compared with the intact lymphocytes. It may be caused by the active replicative processes in BIV-infected cells. It is found that the adhesive properties and the roughness of cell surface of the lymphocytes from cattle with BLV, BIV, BIV-BLV infection increase in 2.3, 3.4, 3.0 times and by 37.3%, 19.2%, 14.8%, respectively, in comparison with the control cows’ lymphocytes. A decrease in the Young's modulus of lymphocytes from cattle with BIV, BLV and BIV-BLV infection of 35.8%, 33.8% and 33.1%, respectively, compared to the control has been shown. It may indicate not only an increase in the cytolemma stiffness, but also an increase in the cell turgor as a result of volume index raising.

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Dmitry A. Artemev , Ekaterina S. Krasnikova, Olga V. Stolbovskaya, Aleksandr V. Krasnikov , Boris B. Kostishko, and Roman V. Radionov "The study of the structural features of the lymphocytes from cattle with and without retroviral infection using atomic force microscopy", Proc. SPIE 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX, 107160G (26 April 2018); https://doi.org/10.1117/12.2317558

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