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26 April 2018 Cytotoxicity evaluation of gold nanoparticles on microalga Dunaliella salina in microplate test system
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Abstract
Gold nanoparticles are intensively studied in biomedicine. Assessment of their biocompatibility is highly important. Currently there is lack of evidence, concerning nanotoxicity of ultrasmall gold nanoparticles < 5 nm. Existing data are rather contradictory. The aim of that study was to evaluate the toxicity of 2 nm colloidal gold, using microalga Dunaliella salina. Cellular barriers of that microalga are very similar to animal cells so it might be considered as a valuable model for nanotoxicity testing. Chlorophyll content as a test–function was used. Spectrophotometric method for chlorophyll determination in vivo in suspensions of D.salina cultures was applied. Calculated EC50 48h value of ionic gold was 25.8 ± 0.3 mg Au/L. EC50 value of phosphine-stabilized gold nanoclusters was 32.2 ±1.1 mg Au/L. It was not possible to calculate EC50 for 15 nm citrate gold nanoparticles, as they were non-toxic at all concentrations tested. These results are confirmed by fluorescent –microscopic monitoring of the same probes. It was shown that 10-fold growth of phosphine-stabilized gold nanoparticles (from 2.3 ± 0.9 nm to 21.1 ± 7.5 nm) led to 7-fold decrease of their toxicity.
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Daniil Chumakov, Artur Prilepskii, Lev Dykman, Boris Khlebtsov, Nikolai Khlebtsov, and Vladimir Bogatyrev "Cytotoxicity evaluation of gold nanoparticles on microalga Dunaliella salina in microplate test system", Proc. SPIE 10716, Saratov Fall Meeting 2017: Optical Technologies in Biophysics and Medicine XIX, 1071617 (26 April 2018); https://doi.org/10.1117/12.2314706
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