26 April 2018 Towards optical control of single blood platelet activation
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Abstract
Blood platelets play a pivotal role in blood coagulation and in other normal and pathological processes. The understanding of fundamental mechanisms underlying their functions is very important for diagnostics and treatment. Single-cell experiments are needed for this purpose, which are complicated by insufficient spatiotemporal precision of conventional activation protocols. We present an approach to trigger single platelet activation optically, without the need of reagent mixing. This is achieved using photolabile compound, which rapidly delivers epinephrine upon UV irradiation. We demonstrated the applicability of the technique to rapidly induce platelet activation for studying dynamics of activation. The presented method may give novel fundamental knowledge about platelet functions and facilitate current research of their ability to deliver drugs to tumors or vascular injury sites.
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Darya V. Spiryova, Oleg Yu. Karmatskih, Alexei Yu. Vorob'ev, Alexander E. Moskalensky, "Towards optical control of single blood platelet activation", Proc. SPIE 10717, Saratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV, 1071722 (26 April 2018); doi: 10.1117/12.2305477; https://doi.org/10.1117/12.2305477
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