You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
5 September 2018Highly sensitive surface-enhanced Raman scattering detection of brodifacoum and 1080 rodenticide in milk
Surface enhanced Raman scattering (SERS) is a powerful technique for food inspection because of its readiness, sensitivity, and minimum sample preparation requirements.1 Milk is a vulnerable target for contamination. In this work, we demonstrate a reliable SERS method for detecting toxins in milk focusing on brodifacoum, an anticoagulant rodenticide and sodium fluoroacetate, also commonly known as 1080. Surface-enhanced Raman spectroscopy is an advanced Raman technique for ultrasensitive detection of chemical and biological species. Liquid milk presents further challenges due to the complex colloidal nature of milk itself; producing much weaker SERS. Therefore, we applied and omniphobic surface platform, which has the potential to deliver near 100% analyte concentration by constant contact angle drying (and therefore no contact line pinning). Such omniphobic SERS substrates, so-called Slippery liquid-infused porous surfaces (SLIPS) were recently reported.2 SLIPSERS method coupled with the dilution of rodenticide spiked milk samples and then extraction with a mixed solvent of methanol: water (3:1) was performed for rodenticide detection.3 All the spectra were taken on an in-house Raman set up based on a Princeton Instruments FERGIE spectrometer using 532 nm excitation wavelength (with 2-3 mW laser power) focused onto the sample using a 40 × 0.65 NA objective. A series of diluted concentrations of each rodenticide ranging from 8- fold dilution to 1600-fold dilution were used to construct a calibration curve. There is a good linear relationship (R2 =0.9897) in this concentration range. A critical challenge in detecting bioanalytes is to achieve high specificity, high throughput, and trace-level detection.4 The approach adopted in this work can be extended to detect various molecules in complex chemical and biological matrices.
Megha Mehta andMark R. Waterland
"Highly sensitive surface-enhanced Raman scattering detection of brodifacoum and 1080 rodenticide in milk", Proc. SPIE 10726, Nanoimaging and Nanospectroscopy VI, 1072618 (5 September 2018); https://doi.org/10.1117/12.2323852
The alert did not successfully save. Please try again later.
Megha Mehta, Mark R. Waterland, "Highly sensitive surface-enhanced Raman scattering detection of brodifacoum and 1080 rodenticide in milk," Proc. SPIE 10726, Nanoimaging and Nanospectroscopy VI, 1072618 (5 September 2018); https://doi.org/10.1117/12.2323852