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18 September 2018 Functional nanoparticles explore a living cell for intracellular dynamics and molecular distribution (Conference Presentation)
Satoshi Kawata
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Proceedings Volume 10740, Molecular Machines; 1074004 (2018) https://doi.org/10.1117/12.2323777
Event: SPIE Organic Photonics + Electronics, 2018, San Diego, California, United States
Abstract
Three-dimensional imaging of molecular distribution in a living cell without labeling or slicing the sample has been a dream of bio-scientists. We have made it possible with use of a gold nanoparticle which explores inside a cell for detecting intracellular molecules based on SERS mechanism [1, 2]. This can be considered as a version of plasmonic tip-enhanced Raman microscopy [3,4], but the tip (as a 50nm particle) is free in a cell. Rather than controlling the nanoparticle motion with a laser trapping technology [5,6], the motion is governed by the cell function. Simultaneous tracking of particle motion provides us a molecular map of organelle transport and lysosomal accumulation. Intracellular environment e.g. pH is also mapped by a surface-functionalized particle. Spatial resolution of this microscope is ~ 65nm (the particle size) and temporal resolution ~50ms. In the presentation, movies of particle motion and a molecular map in a cell will be shown with many other results. 1. J. Ando.et. al., Nano Lett. 11, 5344, 2011 2. K.-C. Huang, et. al., Methods, 68, 348, 2014 3. Y. Inouye and S. Kawata, Opt. Lett. 19, 159, 1994 4. S. Kawata, Y. Inouye, P. Verma, Nat. Photon. 31, 388, 2009 5. S. Kawata, Y. Inouye, T. Sugiura, Jpn. J. Appl. Phys. 33, 1725, 1994 6. T. Sugiura, et. al. Opt. Lett. 22, 1663, 1997
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Satoshi Kawata "Functional nanoparticles explore a living cell for intracellular dynamics and molecular distribution (Conference Presentation)", Proc. SPIE 10740, Molecular Machines, 1074004 (18 September 2018); https://doi.org/10.1117/12.2323777
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KEYWORDS
Nanoparticles
Particles
3D image processing
Molecules
Gold
Laser applications
Microscopy
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