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17 September 2018 Picosecond dynamics in hemes for mitochondrial functional contrast (Conference Presentation)
Erkang Wang, Adam J. Chicco, Randy A. Bartels, Randy A. Bartels, Jesse W. Wilson
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Proceedings Volume 10753, Ultrafast Nonlinear Imaging and Spectroscopy VI; 107530J (2018) https://doi.org/10.1117/12.2320326
Event: SPIE Optical Engineering + Applications, 2018, San Diego, California, United States
Abstract
Pump-probe imaging obtains imaging contrast based on relaxation from a photoexcited state. This has been used as a label-free imaging technique for melanin, hemoglobin, carotenoids, and recently, mitochondrial respiratory chain components. Pump-probe provides a unique opportunity for imaging non-fluorescent molecules, which relax very rapidly (within ~ 200 femtoseconds) to a vibrationally-excited, electronic ground state. In practice, pump and probe wavelengths must be selected that provide good separation between molecules of interest. This is essentially an analytical chemistry application of a technique that is more commonly for physical chemistry. Because of this, much of the pump-probe literature on molecules of interest provide data focused on elucidating energy transfer pathways and molecular structure; predicting from these data which pump/probe wavelengths will work best in an analytical scenario is not straightforward. In this presentation, we discuss general features of pump-probe responses of non-fluorescent molecules, with an eye toward pump/probe wavelength selection. We will focus on spectral shifting and broadening that characterize the vibrationally-excited, electronic ground state, and building a simplified transient absorption model to inform imaging experiments. The end goal of this work is to separate respiratory chain heme proteins and their redox states in a label-free manner. This will lead to new methods for probing mitochondrial function with sub-cellular resolution.
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Erkang Wang, Adam J. Chicco, Randy A. Bartels, Randy A. Bartels, and Jesse W. Wilson "Picosecond dynamics in hemes for mitochondrial functional contrast (Conference Presentation)", Proc. SPIE 10753, Ultrafast Nonlinear Imaging and Spectroscopy VI, 107530J (17 September 2018); https://doi.org/10.1117/12.2320326
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KEYWORDS
Molecules
Chemistry
Molecular energy transfer
Absorption
Energy transfer
Eye
Eye models
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