In this paper, the fluorescence quantum efficiency of 3 different samples at atmosphere was compared, and the electrospray ionization source was selected for its soft ionization characteristics. The ionization method did not spoil the fluorophore of the sample, and the drift tube of ion mobility spectrometry (IMS) was used for ions transport and desolvation. The ionization source was on the one side of the drift tube and the test point was on the other side. The paths of excited laser and emission light were orthogonal at the test point. Meanwhile, stable ions flowed through the drift tube. The emission light was captured by the camera, which was coupled with a long-wave pass filter. The test samples were Rhodamine 6G，Rhodamine B and amino copper indium sulfide quantum dots of the same mass fraction. The energy of excited laser was between 30 mW and 150 mW. Then the results showed that the emission intensity was proportional to the laser power in gas phase, and the sort of the fluorescence quantum efficiency was the quantum dots>Rhodamine 6G>Rhodamine B.
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