In vivo monitoring optical clearing process of skin using two-photon microscopy

Lu Deng; Wei Feng; Tingting Yu; Dan Zhu

doi:10.1117/12.2502503

23 October 2018 In vivo monitoring optical clearing process of skin using two-photon microscopy

Lu Deng, Wei Feng, Tingting Yu, Dan Zhu

Author Affiliations +

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108203E (2018) https://doi.org/10.1117/12.2502503
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

The skin is the largest organ in the human body, and it is an important window for optical imaging and diagnosis. However, the turbid characteristics of skin limits the penetration of light. Skin optical clearing technique provides an important tool for improving optical imaging performance. However, study on mechanism for skin optical clearing was mostly based on ex vivo skin experiments, the in vivo skin optical clearing process still needs further exploration. In this work, we can in vivo monitor the changes in skin during treatment of skin optical clearing agent by using two-photon imaging. For untreated skin, collagen has a strong second harmonic generation (SHG) signal, the structure of epidermis and collagen is relatively complete. After treatment of skin optical clearing agent, the SHG signal of collagen is significantly weakened. After optical clearing agent removed and saline treated, the skin can recover to initial state obviously, and the SHG is greatly enhanced, even is stronger than initial level. The results indicated that the treatment of optical clearing agents (OCA) can induce the structural change of skin, which lead to skin transparent consequently, and optical clearing agent–induced decrease of collagen SHG is invertible by recovery with treatment of physiological saline. This work is very helpful for understanding the in vivo skin optical clearing process, which is of great significant to revealing the mechanism of in vivo skin optical clearing.

Citation Download Citation

Lu Deng, Wei Feng, Tingting Yu, and Dan Zhu "In vivo monitoring optical clearing process of skin using two-photon microscopy", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108203E (23 October 2018); https://doi.org/10.1117/12.2502503

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Optical clearing

Collagen

Second-harmonic generation

In vivo imaging

3D image processing

Two photon imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years