CONFERENCE PROCEEDINGS
Advanced Search >
Home > Proceedings > Volume 10834 > Article
Translator Disclaimer
7 September 2018 Qualitative characterization of collagen hydrogel membranes using digital holographic microscopy and SHG microscopy
J. M. Flores-Moreno; Birzabith Mendoza-Novelo; Laura E. Castellano; Manuel H. De la Torre-I; Ma. Del Socorro Hernández; Carlos Pérez-López; Fernando Mendoza-Santoyo
Author Affiliations +
Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 108340J (2018) https://doi.org/10.1117/12.2319422
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland
Abstract
Crosslinked collagen hydrogel membranes are widely used in engineering tissue to promote the performance of biological processes involved in the healing of wounds, mainly in the presence of chronical diseases such as diabetes. One of the standard techniques used in biology to measure mechanical properties of hydrogels and tissues is based on a methodology called rheology. Rheological studies consist on the measurement of stress, strain and the ratio of stress to strain of several biological membranes to determine their viscoelastic properties. In this research work we propose as a proof of concept the use of digital holographic microscopy (DHM) and second harmonic generation (SHG) microscopy to compare qualitatively some basic image properties of collagen hydrogel membranes. Once demonstrated that this comparison is equivalent, we study under controlled excitation the biomechanical properties of collagen membranes by means of the analysis of the optical phase that results from comparing two consecutives holograms from a reference and deformed state. At this stage of characterization, an in depth study between rheological and holographic results will be performed in the near future.
© (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
J. M. Flores-Moreno, Birzabith Mendoza-Novelo, Laura E. Castellano, Manuel H. De la Torre-I, Ma. Del Socorro Hernández, Carlos Pérez-López, and Fernando Mendoza-Santoyo "Qualitative characterization of collagen hydrogel membranes using digital holographic microscopy and SHG microscopy", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108340J (7 September 2018); https://doi.org/10.1117/12.2319422
PROCEEDINGS
 6 PAGES
DOWNLOAD PDF SAVE TO MY LIBRARY
SHARE
GET CITATION
< Previous Article
|
Next Article >
Advertisement
Advertisement
KEYWORDS
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top