Speckle imaging for monitoring the growth kinetics of Bacillus thuringiensis

Hadi Loutfi; Hassan Badawi; Fabrice Pellen; Bernard Le Jeune; Roger Lteif; Mireille Kallassy; Guy Le Brun; Marie Abboud

doi:10.1117/12.2318678

7 September 2018 Speckle imaging for monitoring the growth kinetics of Bacillus thuringiensis

Hadi Loutfi, Hassan Badawi, Fabrice Pellen, Bernard Le Jeune, Roger Lteif, Mireille Kallassy, Guy Le Brun, Marie Abboud

Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 108341C (2018) https://doi.org/10.1117/12.2318678
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland

Abstract

This paper presents an application of laser speckle imaging method to characterize the kinetic growth of Bacillus thuringiensis (Bt). Numbers of parameters, such as speckle grain size and spatial contrast, are considered in order to characterize the culture medium and to monitor in real time the fermentation process. We show that the grain size and the contrast of the speckle image decrease with the increase of the cells concentration. The correlation of speckle results with optical density measurements shows the effectiveness of dynamic speckle for real-time monitoring of Bt cells growth kinetics.

Citation Download Citation

Hadi Loutfi, Hassan Badawi, Fabrice Pellen, Bernard Le Jeune, Roger Lteif, Mireille Kallassy, Guy Le Brun, and Marie Abboud "Speckle imaging for monitoring the growth kinetics of Bacillus thuringiensis", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 108341C (7 September 2018); https://doi.org/10.1117/12.2318678

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