Scanning errors in holographic tomography

Arkadiusz Kuś; Sarah Mues; Björn Kemper

doi:10.1117/12.2319628

7 September 2018 Scanning errors in holographic tomography

Arkadiusz Kuś, Sarah Mues, Björn Kemper

Proceedings Volume 10834, Speckle 2018: VII International Conference on Speckle Metrology; 1083421 (2018) https://doi.org/10.1117/12.2319628
Event: SPECKLE 2018: VII International Conference on Speckle Metrology, 2018, Janów Podlaski, Poland

Abstract

In holographic tomography obtaining projections is the key part of the measurement process that enables the 3D refractive index reconstruction. The error of the illumination angle can significantly influence the reconstruction quality and alter the result degrading the reliability of the reconstruction. Thus, in this paper the impact of the scanning errors in limited angle holographic tomography with respect to two reconstruction algorithms is analyzed. The simulated errors are compared to the errors identified in the experimental system. The reconstruction errors are verified using a paramecium cell phantom at the simulation stage and with a biological object, namely a macrophage cell in the experimental part. The experimental system presented in the paper exhibits maximum expected measurement errors found in galvanometer-mirror-based holographic tomography setups.

Citation Download Citation

Arkadiusz Kuś, Sarah Mues, and Björn Kemper "Scanning errors in holographic tomography", Proc. SPIE 10834, Speckle 2018: VII International Conference on Speckle Metrology, 1083421 (7 September 2018); https://doi.org/10.1117/12.2319628

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