Most of the traditional camouflage target detection rely on the human eyes to make a simple magnification observation through white light instruments, but the detection result is usually not good. The hyperspectral instrument can acquire spectral images of a certain band range, and then obtain a continuous spectral curve of the object. In general, there are differences in spectral curves between different objects. With hyperspectral detection, the camouflaging characteristics of the camouflaged target in different backgrounds are different. In order to obtain the best camouflage effect, the difference between camouflage clothing and different background in hyperspectral images were analyzed. The difference between disguised camouflage clothing and backgrounds were compared by the spectral amplitude and the change trend of the spectral shape. Based on Euclidean distance, spectral information divergence, spectral angle and spectral gradient angle, the camouflage effect of camouflaged target in the background were evaluated from spectral reflectance, information theory, spectral angle and spectral gradient respectively. Camouflage clothing and light green plastic fake turf, dark green plastic fake turf, real turf, camouflage raincoat and old camouflage backgrounds were compared. Overall, camouflage clothing in the real grass camouflage effect is best, which conforms to design intention, and it can provide a good camouflage for military operations. At the same time, the experiment also shows that if the disguised target detection is only considered from a certain aspect, it may get wrong results. Therefore, in the disguise detection, it is necessary to comprehensively consider the detection target from various aspects.