Paper
28 February 2019 Confocal Raman microscopy and non linear microscopy to study human enamel incipient lesions
Rand Al-Obaidi, Hervé Tassery, Thierry Cloitre, Csilla Gergely, Frédéric Cuisinier, Hamideh Salehi
Author Affiliations +
Proceedings Volume 10857, Lasers in Dentistry XXV; 108570C (2019) https://doi.org/10.1117/12.2508306
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
Incipient enamel lesions represent an early phase of dental caries. They can be produced in-vitro by pH cycling. Raman spectral maps showed that the higher the organic/mineral ratio in the demineralized enamel, the lower is the intensity of mineral content in the same zone. Moreover, the evident red shift of the two-photon excited fluorescence (2PEF) spectrum from carious enamel, compared to that from sound enamel is typical of carious lesion formation. Second harmonic generation (SHG) signal was also observed in the lesion zone. The obtained results demonstrate a great potential for Raman, 2PEF and SHG photonic signals in diagnostic of incipient carious lesions.
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Rand Al-Obaidi, Hervé Tassery, Thierry Cloitre, Csilla Gergely, Frédéric Cuisinier, and Hamideh Salehi "Confocal Raman microscopy and non linear microscopy to study human enamel incipient lesions", Proc. SPIE 10857, Lasers in Dentistry XXV, 108570C (28 February 2019); https://doi.org/10.1117/12.2508306
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KEYWORDS
Confocal microscopy

Raman spectroscopy

Microscopy

Multiphoton microscopy

Second-harmonic generation

Luminescence

Crystals

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