Multiphoton imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism in vivo

Hsu-Cheng Huang; Chih-Ju Lin; Sheng-Lin Lee; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.2507347

28 February 2019 Multiphoton imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism in vivo

Hsu-Cheng Huang, Chih-Ju Lin, Sheng-Lin Lee, Hsuan-Shu Lee, Chen-Yuan Dong

Proceedings Volume 10859, Visualizing and Quantifying Drug Distribution in Tissue III; 108590B (2019) https://doi.org/10.1117/12.2507347
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We used intravital multiphoton microscopy to study the recovery of hepatobiliary metabolism following carbon tetrachloride (CCl₄) induced hepatotoxicity in mouse. Our images were processed by a first order kinetic model to generate rate constant resolved images of the mouse liver. At Day 14 following induction, a restoration of the mouse hepatobiliary function was found. Our approach allows the study of the response of hepatic functions to chemical agents in real time and is useful for studying pharmacokinetics of drug molecules through optical microscopic imaging.

Citation Download Citation

Hsu-Cheng Huang, Chih-Ju Lin, Sheng-Lin Lee, Hsuan-Shu Lee, and Chen-Yuan Dong "Multiphoton imaging of the toxic effect of carbon tetrachloride on hepatobiliary metabolism in vivo", Proc. SPIE 10859, Visualizing and Quantifying Drug Distribution in Tissue III, 108590B (28 February 2019); https://doi.org/10.1117/12.2507347

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