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4 March 2019 (Re)defining sensitivity of chemical imaging
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Abstract
To successfully develop and manufacture a generic drug product, the latter is expected to be bioequivalent to its referencelisted drug, i.e. to show no significant difference in the rate and extent of absorption of the active pharmaceutical ingredient. Optical spectroscopy methods based on vibrational spectroscopy imaging have recently attracted significant attention as potentially viable approaches to quantify drugs’ pharmacokinetics in vivo. However, substantial hurdles still exist due to signal interference from surrounding tissues, significant attenuation of signal in the depth of tissue to optical absorption and scattering, and a lack of quantifiable ways of assessing the signal generated from a drug compound in the depth of a tissue. In this report, we evaluated the major challenges of quantification and sensitive and reproducible analysis of drug distribution in tissues using Raman spectroscopy. Specific attention is given to the noise assessment, which affects both the sensitivity and reproducibility of data.
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sean P. O'Connor, Joshua Lalonde, Dawson T. Nodurft, and Vladislav V. Yakovlev "(Re)defining sensitivity of chemical imaging", Proc. SPIE 10859, Visualizing and Quantifying Drug Distribution in Tissue III, 108590H (4 March 2019); https://doi.org/10.1117/12.2510974
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