Validation of tissue optical properties measurement using diffuse reflectance spectroscopy (DRS)

Yi-Hong Ong; Yihua Zhu; Timothy C. Zhu

doi:10.1117/12.2513558

28 February 2019 Validation of tissue optical properties measurement using diffuse reflectance spectroscopy (DRS)

Yi-Hong Ong, Yihua Zhu, Timothy C. Zhu

Proceedings Volume 10860, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVIII; 108600D (2019) https://doi.org/10.1117/12.2513558
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The effectiveness of photodynamic treatment depends on several factors including an accurate knowledge of optical properties of the tissue to be treated. Transmittance and diffuse reflectance spectroscopic techniques are commonly used to determine tissue optical properties. Although transmittance spectroscopy technique is accurate in determining tissue optical properties, it is only valid in an infinite medium and can only be used for interstitial measurements. Diffuse reflectance spectroscopy, on the other hand, is easily adapted to most tissue geometries including skin measurements that involve semi-infinte medium. However, the accuracy of the measured optical properties can be affected by uncertainty in the measurements themselves and/or due to the uncertainty in the fitting algorithm. In this study, we evaluate the accuracy of optical properties determination using diffuse reflectance spectroscopy implemented using a contact probe setup. We characterized the error of the optical properties fitted using two fitting algorithms, a wavelength wise fitting algorithm and a full reflectance spectral fitting algorithm. By conducting systematic investigation of the measurements and fitting algorithm of DRS, we gained an understanding of the uncertainties in the measured optical properties and outlined improvement measures to minimize these errors.

Conference Presentation

Citation Download Citation

Yi-Hong Ong, Yihua Zhu, and Timothy C. Zhu "Validation of tissue optical properties measurement using diffuse reflectance spectroscopy (DRS)", Proc. SPIE 10860, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVIII, 108600D (28 February 2019); https://doi.org/10.1117/12.2513558

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