Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

Joshua W. Lalonde; Sean P. O'Connor; Gary D. Noojin; Cherry C. Gonzales; Vladislav V. Yakovlev; Michael L. Denton

doi:10.1117/12.2508684

7 March 2019 Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light

Joshua W. Lalonde, Sean P. O'Connor, Gary D. Noojin, Cherry C. Gonzales, Vladislav V. Yakovlev, Michael L. Denton

Author Affiliations +

Proceedings Volume 10861, Mechanisms of Photobiomodulation Therapy XIV; 108610I (2019) https://doi.org/10.1117/12.2508684
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Photobiomodulation, also known as low level laser therapy (LLLT), is a technique that uses light in the red and near infrared (NIR) range (600-900 nm) to elicit a clinically beneficial physiological change in tissue. This physiological change is thought to begin in the mitochondria by altering the metabolic rate for the electron transport chain (ETC). Resonance Raman spectroscopy at 532 nm was used to determine the reduction/oxidation (redox) state of cytochrome c in isolated mitochondria after undergoing LLLT. Mitochondria from hTERT-RPE1 cells were isolated and placed in glutamate buffer and then exposed to violet (405 nm) or red (635 nm) light. The resonance Raman spectrum of the cytochrome c redox state before and after light illumination was measured. This gives us an insight into the types of metabolic changes that occurs within the mitochondria while being illuminated by light during photobiomodulation.

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Joshua W. Lalonde, Sean P. O'Connor, Gary D. Noojin, Cherry C. Gonzales, Vladislav V. Yakovlev, and Michael L. Denton "Measuring cytochrome c redox state using resonance Raman spectroscopy to determine metabolic rates in electron transport chain when exposed to light", Proc. SPIE 10861, Mechanisms of Photobiomodulation Therapy XIV, 108610I (7 March 2019); https://doi.org/10.1117/12.2508684

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