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22 May 2019 Measuring microdose ABY-029 fluorescence signal in a primary human soft-tissue sarcoma resection
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Abstract
Near-infrared (NIR) fluorescence combined with targeting epidermal growth factor receptor (EGFR) overexpression for surgical guidance in many cancers is gaining momentum. ABY-029 is an anti-EGFR Affibody molecule conjugated to IRDye 800CW that is FDA approved as an exploratory Investigational New Drug (eIND 122681). ABY-029 has a short plasma half-life (~15-20 minutes), which allows for administration of the imaging agent and excision surgery to occur on the same day unlike fluorescent antibodies. This fast tissue clearance may provide the means necessary to achieve clinically relevant tumor-to-normal tissue contrast levels using microdosing administration schemes. Pre-clinical studies have indicated that tumor-to-normal tissue contrast peaks between 4-8 hours depending on EGFR expression. Additionally, the No Observable Adverse Effect Level (NOAEL) was determined in pre-clinical toxicity studies to be 1,000X the microdose, or 30 micromole, whereas mild adverse events are common in antibody imaging studies. A number of promising first-in-human clinical Phase 0 trial microdose evaluation of ABY-029 have been initiated for recurrent glioma, soft-tissue sarcoma, and head and neck cancers (NCT0290925, NCT03154411, and NCT03282461, respectively). Here, we provide an update on our experience using ABY-029 for surgical resection at microdose levels and describe tissue contrast and correlation of ABY-029 fluorescence to EGFR tissue expression. Current progress indicates that moderate TBRs (~3-5) are observable at the microdose administration level but increased administration doses (3X and 6X microdoses) are being investigated. In addition, ex vivo tissue fluorescence is highly correlated to EGFR staining in both intensity and spatial localization.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kimberley S. Samkoe, Hira Shahzad Sardar, Jason Gunn, Joachim Feldwisch, Konstantinos Linos, Eric Henderson, Brian Pogue, and Keith Paulsen "Measuring microdose ABY-029 fluorescence signal in a primary human soft-tissue sarcoma resection", Proc. SPIE 10862, Molecular-Guided Surgery: Molecules, Devices, and Applications V, 1086212 (22 May 2019); https://doi.org/10.1117/12.2510935
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