Pure proteinaceous high-aspect-ratio microstructures made by femtosecond laser multiphoton cross-linking

D. Serien; K. Sugioka

doi:10.1117/12.2509131

4 March 2019 Pure proteinaceous high-aspect-ratio microstructures made by femtosecond laser multiphoton cross-linking

D. Serien, K. Sugioka

Proceedings Volume 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII; 1087504 (2019) https://doi.org/10.1117/12.2509131
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We report the fabrication of pure proteinaceous high-aspect-ratio microstructures made by femtosecond (fs) laser multiphoton cross-linking for different variants of serum albumin, mouse serum albumin (MSA) and bovine serum albumin (BSA). Recently, use of laser direct writing to fabricate 3D microstructures made of protein has enabled applications towards cell culture and pH-actuation. This method employs the direct exposure of a focused fs laser into aqueous solution containing protein molecules enabling fabrication of solid protein structures by cross-linking protein molecules along the arbitrary exposure pathway. Preliminary high-aspect-ratio microstructures are examined by scanning electron microscopy. We report mechanical analysis of different structure concepts by finite element analysis to approach high-aspect-ratio design despite 1 MPa-Young’s modulus of protein hydrogel. Protein as a biomolecule and relevant material group for pharmaceutical developments is a promising choice for biomedical device fabrication. Fabrication of biomedical MEMS based on biomaterials such as protein might directly become important for implant and biomedical technologies. (154 of 200).

Citation Download Citation

D. Serien and K. Sugioka "Pure proteinaceous high-aspect-ratio microstructures made by femtosecond laser multiphoton cross-linking", Proc. SPIE 10875, Microfluidics, BioMEMS, and Medical Microsystems XVII, 1087504 (4 March 2019); https://doi.org/10.1117/12.2509131

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