Optimized 3DISCO for imaging of heme-rich tissues by decolorization

Yilin Ma; Tingting Yu; Jianyi Xu; Peng Wan; Yisong Qi; Wei Feng; Dan Zhu

doi:10.1117/12.2512690

1 March 2019 Optimized 3DISCO for imaging of heme-rich tissues by decolorization

Yilin Ma, Tingting Yu, Jianyi Xu, Peng Wan, Yisong Qi, Wei Feng, Dan Zhu

Proceedings Volume 10877, Dynamics and Fluctuations in Biomedical Photonics XVI; 108770P (2019) https://doi.org/10.1117/12.2512690
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Tissue optical clearing technique plays an important role in three-dimensional (3D) visualization of intact organs or rodent bodies. As a typical organic-solvent based clearing method, 3DISCO renders the turbid tissues transparent by the strategy of minimizing light scattering, and possesses the advantages of high transparency and substantial size reduction, which could facilitate imaging of large-volume tissues. However, for heme-rich tissues, such as spleen, liver, embryo and clinical biopsy samples, tissue transparency and image quality by 3DISCO are limited due to the strong absorption ability of hemes. To address this problem, we proposed an optimized clearing method by introducing decolorization to modify the original 3DISCO protocol. The results showed that the optimized protocol could enhance the transparency of the heme-rich tissues and facilitate high-resolution imaging inside these tissues combining with optical microscopies.

Conference Presentation

Citation Download Citation

Yilin Ma, Tingting Yu, Jianyi Xu, Peng Wan, Yisong Qi, Wei Feng, and Dan Zhu " Optimized 3DISCO for imaging of heme-rich tissues by decolorization", Proc. SPIE 10877, Dynamics and Fluctuations in Biomedical Photonics XVI, 108770P (1 March 2019); https://doi.org/10.1117/12.2512690

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