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27 February 2019 Investigation of the thermal properties of biological cells using a frequency domain photoacoustic microscope
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Abstract
A photoacoustic sensor for studying the thermal properties of a single biological cell was developed. The sensor used an aluminium foil for heating the sample which enables the study of unstained samples. The PA sensor was developed to work in the heat transmission mode configuration by positioning the detector at the opposite side with respect to heated side of the sample. Rosencwaig’s theoretical model for an open PA cell configuration functional in a heat transmission mode was applied to study the thermal diffusivity of biological cells. MCF7 cells were used in this studied. We measured the thermal diffusivity of 3 cells using the sensor. The average measured thermal diffusivity of MCF7 cells was 0.05 mm2/s. The PA sensor allows the spatial mapping of the cell thermal diffusivity and can be used to measure thermal properties of cells in various phases of the cell cycle. It can also be used to measure the effective properties of cells when they have internalized various sensors (e.g. plasmonic nanoparticles).
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Krishnan Sathiyamoorthy and Michael C. Kolios "Investigation of the thermal properties of biological cells using a frequency domain photoacoustic microscope", Proc. SPIE 10878, Photons Plus Ultrasound: Imaging and Sensing 2019, 108785X (27 February 2019); https://doi.org/10.1117/12.2510511
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