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The papers in this volume were part of the technical conference cited on the cover and title page. Papers were selected and subject to review by the editors and conference program committee. Some conference presentations may not be available for publication. Additional papers and presentation recordings may be available online in the SPIE Digital Library at SPIEDigitalLibrary.org. The papers reflect the work and thoughts of the authors and are published herein as submitted. The publisher is not responsible for the validity of the information or for any outcomes resulting from reliance thereon. Please use the following format to cite material from these proceedings: Author(s), “Title of Paper,” in Multiphoton Microscopy in the Biomedical Sciences XIX, edited by Ammasi Periasamy, Peter T. C. So, Karsten König, Proceedings of SPIE Vol. 10882 (SPIE, Bellingham, WA, 2019) Seven-digit Article CID Number. ISSN: 1605-7422 ISSN: 2410-9045 (electronic) ISBN: 9781510624061 ISBN: 9781510624078 (electronic) Published by SPIE P.O. Box 10, Bellingham, Washington 98227-0010 USA Telephone +1 360 676 3290 (Pacific Time)· Fax +1 360 647 1445 Copyright © 2019, Society of Photo-Optical Instrumentation Engineers. Copying of material in this book for internal or personal use, or for the internal or personal use of specific clients, beyond the fair use provisions granted by the U.S. Copyright Law is authorized by SPIE subject to payment of copying fees. The Transactional Reporting Service base fee for this volume is $18.00 per article (or portion thereof), which should be paid directly to the Copyright Clearance Center (CCC), 222 Rosewood Drive, Danvers, MA 01923. Payment may also be made electronically through CCC Online at copyright.com. Other copying for republication, resale, advertising or promotion, or any form of systematic or multiple reproduction of any material in this book is prohibited except with permission in writing from the publisher. The CCC fee code is 1605-7422/19/$18.00. Printed in the United States of America by Curran Associates, Inc., under license from SPIE. Publication of record for individual papers is online in the SPIE Digital Library.
Paper Numbering: Proceedings of SPIE follow an e-First publication model. A unique citation identifier (CID) number is assigned to each article at the time of publication. Utilization of CIDs allows articles to be fully citable as soon as they are published online, and connects the same identifier to all online and print versions of the publication. SPIE uses a seven-digit CID article numbering system structured as follows:
AuthorsNumbers in the index correspond to the last two digits of the seven-digit citation identifier (CID) article numbering system used in Proceedings of SPIE. The first five digits reflect the volume number. Base 36 numbering is employed for the last two digits and indicates the order of articles within the volume. Numbers start with 00, 01, 02, 03, 04, 05, 06, 07, 08, 09, 0A, 0B…0Z, followed by 10-1Z, 20-2Z, etc. Alismail, Ayman, 31 Alspaugh, Gregory R., 2A Andreoni, Alessio, 0O, 2A Antolovic, Ivan Michel, 0M Ardelean, Andrei, 0Y Barbieri, Beniamino, 0I, 34 Barbiero, Gaia, 31 Barnard, Edward S., 0F Basunia, Razeen, 2L Batista, Ana, 1A Beavil, Andrew J., 10 Beavil, Rebecca L., 10 Becker, Wolfgang, 06, 09, 0B Benirschke, David, 2H Bergmann, Axel, 06 Bergmann, Axel, 0B Bernerd, F., 0A Bi, Sarah, 2P Birngruber, Reginald, 2F Blaszkiewicz, Magdalena, 18 Bobrov, Nikolai V., 2K Böhm, Ingo, 0S Bornschlögl, T., 0A Borrego, Eric D., 2P Borri, Paola, 1P Börsch, Michael, 0W Boulade, M., 0A Braun, Lukas, 0B Breeding, Patrick W., 18 Breunig, Hans Georg, 0F, 1A Brinkmann, Ralf, 2F Brizion, S., 0A Bruschini, Claudio, 0M, 0Y Chan, James W., 13 Chan, Kin F., 2J Chang, Che-Wei, 13 Charbon, Edoardo, 0M, 0Y Chen, Mingzhou, 1G Chen, Xiaohong, 30 Chen, Xin, 2J Chen, Xun, 2L Chen, Yang-Fang, 12 Chen, Yanpin, 30 Chen, Yuan-I, 2P Cheng, Ji-Xin, 2B, 2D, 2E Chiamvimonvat, Nipavan, 13 Chung, Pei-Hua, 10 Clayton, Andrew, 2Z Coleman, Jason E., 30 Cornell, Bethan, 10 Coskun, Ulas C., 34 Crnjar, Alessandro, 10 Davydova, Diana, 0G DeGrip, Willem J., 2D Deng, Fengyuan, 2B Dholakia, Kishan, 1G Diaspro, Alberto, 0I Ding, Changqin, 17 Dong, Chen-Yuan, 12, 1B, 35 Draxinger, Wolfgang, 2F Dreiss, Cécile A., 10 Dudenkova, Varvara V., 09, 0G, 2K Economou, Augoustina M., 10 Eibl, Matthias, 2F Elagin, Vadim, 0G Errington, Rachel, 1P Escobet-Montalbán, Adrià, 1G Evans, Conor L., 2J Fast, Alexander, 2J Fattahi, Hanieh, 31 Fedoseeva, Vera V, 09 Friedenauer, Axel, 0U Galey, J.-B., 0A Gannon, Steven, 2L Garanina, Oksana, 0G Gottschall, Thomas, 2F Greisberger, Isabel, 0U Gubarkova, Ekatarina, 0G Gugel, Hilmar, 0S Guldner, Ian H., 2H Hakert, Hubertus, 2F Han, Won Sub, 1J Hao, Yunqi, 30 Harling, Mitchell, 2A Heitkamp, Thomas, 0W Hellerer, Thomas, 0U, 2S Hermsmeier, Maiko, 2J Hilleringmann, Markus, 2S Hirvonen, Liisa M., 10 Hovhannisyan, Vladimir A., 1B Howard, Scott S., 2H Hsueh, Chiu-Mei, 35 Huang, Hsu-Cheng, 1B Huang, Kai-Chih, 2D, 2E Huang, Lin, 2G Huang, Shao-Po, 2P Huang, Yao-De, 1B, 35 Huber, Robert, 2F Hussain, Syed Ali, 31 Hwang, Wonsang, 1J Ishitsuka, Yuji, 0I James, Arjuna L., 10 Jeong, Sinyoung, 2J Kalinina, Sviatlana, 07, 32 Kang, Mingu, 1J Kao, Hillary K. J., 13 Karpf, Sebastian Nino, 2F Karuna, Arnica, 1P Kavanagh, Thomas, 2C Khalil, Andre, 18 Kim, Dongeun, 1J Kim, Dug Young, 1J Kim, Minsu, 2I Kiselev, Nikolai M., 2K Kisileva, Elena B., 09 Klemenova, Irina, 0G Knutson, Jay R., 0O, 2A Kolb, Jan Philip, 2F König, Aisada, 1A König, Karsten, 0F, 1A Krausz, Ferenc, 31 Kuo, Yu-An, 2P Kuznetsova, Daria S., 2K Lajevardipour, Alireza, 2Z Langbein, Wolfgang, 1P Lanzanò, Luca, 0I Le Marois, Alix, 10 Ledford, Hannah A., 13 Lee, Hsuan-Shu, 1B, 35 Lee, Hyeon Jeong, 2D Lee, Sang Yoon, 1J Lee, Sheng-Lin, 12, 1B, 35 Levitt, James A., 10 Li, Junjie, 2E Li, Yang, 2L Liao, Shih-Chu (Jeff), 34 Lieu, Deborah K., 13 Limpert, Jens, 2F Lin, Chih-Ju, 1B Lin, Haonan, 2B Littleton, Brad, 2C Liu, Yen-Liang, 2P Lorenz, Christian D., 10 Lukina, Maria, 09 Mamaeva, Natalia, 2D Masia, Francesco, 1P Mei, Gaoxiang, 2D Meyer, Tobias, 2F Michalet, Xavier, 0M, 0Y Mohseni, Mojtaba, 2S Molteni, Carla, 10 Moon, Sucbei, 1J Mrowka, Ralf, 0W Müller, Philippe-Fabian, 0B Murphy, Michael, 2A Nadeau, Elizabeth, 2L Nedbal, Jakub, 10 Ness, Stefan, 2S Neugart, Felix, 0S Nguyen, Trung D., 2P Nichols, Evan L., 2H Nie, Yu, 2C Orlinskaya, Natalia, 0G Ortiz-Zapater, Elena, 10 Parsons, Maddy, 10 Pellegrini, Vincent, 2L Pena, A.-M., 0A Penjweini, Rozhin, 0O, 2A Planel, E., 0A Polzer, Christoph, 0U, 2S Popp, Jürgen, 2F Rädler, Joachim, 2S Reeß, K., 07 Reeves, Russell, 2L Reuter, Stefanie, 0W Richards, David, 2C Roarke, Branden, 0O Rodimova, Svetlana A., 2K Rothschild, Kenneth J., 2D Rück, Angelika, 07, 32 Sackett, Dan L., 0O Saini, Neal, 2L Schaefer, Patrick, 32 Schmitz, Rebecca, 0C Schweinberger, Wolfgang, 31 Selvin, Paul R., 0I Shah, Priyank, 2C Shah, Sunil, 0I, 34 Shcheslavskiy, Vladislav I., 09 Shirmanova, Marina V., 09 Shlivko, Irena, 0G Sielaff, Hendrik, 0W Simpson, Garth J., 17 Skala, Melissa C., 0C Skrobol, Christoph, 0U Smietana, Stefan, 06 Smirnov, Aleksander V., 2A Smith, Cody J., 2H Steinmark, I. Emilie, 10 Strub, Marie-Paule, 0O, 2A Sturzenbaum, Stephen, 2C Suarez Ibarrola, Rodrigo, 0B Suhling, Klaus, 10 Sun, Yuansheng, 0I, 34 Tang, Shuo, 2G Teijeiro-Gonzalez, Yurema, 10 Teng, Kai-Wen, 0I Tilbury, Karissa B., 18 Tissot, N., 0A Tortarolo, Giorgio, 0I Townsend, Kristy, 18 Ulcickas, James R. W., 17 Ulku, Arin Can, 0M, 0Y Ursprung, Benedikt, 0F Vicidomini, Giuseppe, 0I von Einem, Bjorn, 07, 32 Walsh, Alex J., 0C Walsh, Christine, 0C Wang, Haochuan, 31 Wang, Mengran, 2I Wang, Wei-Hsiang, 1B Watkins, Beckham, 2L Weiss, Shimon, 0M Weng, Daniel, 2F Westphal, Anika, 0W Wijesinghe, Philip, 1G Wiltshire, Marie, 1P Wolfring, Bernhard, 0U Won, Youngjae, 1J Xia, Fei, 2I Xie, Huikai, 30 Xu, Chris, 2I Yahioglu, Gokhan, 10 Yamamoto, Akira, 2J Yao, Hai, 2L Ye, Tong, 2L Yeh, Hsin-Chih, 2P Zagainov, Vladimir E., 2K Zagaynova, Elena V., 09, 0G, 2K Zhang, Chi, 2B, 2E Zhang, Siyuan, 2H Zhang, Xiao-Dong, 13 Zhang, Yide, 2H Zhou, Liang, 30 Zong, Cheng, 2B Conference CommitteeSymposium Chairs
Symposium Co-chairs:
Program Track Chairs
Conference Chairs
Conference Program Committee
Session Chairs
Poster Session Holly Aaron, University of California, Berkeley (United States) Michael Börsch, Universitätsklinikum Jena (Germany) Alberto Diaspro, Istituto Italiano di Tecnologia (Italy) Kevin W. Eliceiri, University of Wisconsin-Madison (United States) Lingyan Shi, Columbia Univ. (United States) IntroductionMultiphoton microscopy has been established as the 3D imaging method of choice for studying living biomedical specimens from single cells and whole animals to patients with submicron resolution. 29 years have passed since the realization of two-photon laser scanning microscopy. The ever-expanding scope of applications and the continuing instrumental innovations require a forum where new ideas can be exchanged and presented. Our conference at the SPIE BIOS2019 meeting continues to address this need. This was the 19th year of this conference and we started our conference with four Keynote lectures from leaders in the field of metabolism:
For the 7th year, we were extremely pleased to have the JenLab Young Investigator Award, in addition to our regular poster awards. JenLab Young Investigator Award is sponsored by JenLab GmbH (Germany). The award selection committee included Drs. Arnd Krueger, Spectra Physics, a division of MKS Instruments (United States), Paul Campagnola, University of Wisconsin-Madison (United States), Conor Evans, Massachusetts General Hospital (United States), Alberto Diaspro, Istituto Italiano di Tecnologia (Italy), Holly Aaron, University of California at Berkeley (United States), and the three conference chairs (Ammasi, Karsten and Peter). The selection process reviewed seven abstracts, manuscripts and five-minute oral presentations. Two finalists were selected for 15-minute oral presentation after their five-minute oral presentation. The two finalists were:
The review panel selected Dr. Yide Zhang, Univ. of Notre Dame (United States), as the winner of the JenLab Young Investigator Award 2017. The winner received a certificate and $1500. The runner-up received $500 award. The award was presented by Prof. Dr. Karsten König, Saarland University (Germany), and President and Founder of JenLab GmbH (Germany). For 19 years in a row, the conference organized poster awards for the students and postdoctoral fellows. The poster award was donated by all the conference sponsors as acknowledged at the bottom of the page. The review panelists were, Holly Aaron, University of California at Berkeley (United States), Lingyan Shi, Columbia University (United States), Michael Börsch, Universitätsklinikum Jena (Germany), and Conor Evans, Massachusetts General Hospital (United States). The 4 poster award winners were:
Some of the most valuable contributions in this volume are articles written by highly experienced practitioners of multiphoton microscopy. They have enumerated the most important considerations in designing multiphoton microscopes and imaging experiments. Further, updates on the state-of-the-art commercial multiphoton microscope systems are presented. This volume also includes articles describing some recent advances in major multiphoton microscope components and applications including laser light sources, ultra-fast optics, filters, FRET, FLIM, FCS, Raman, CARS, SRS and Coherent Raman microscopy and spectroscopy, single molecule, endoscopy, In vivo/Intravital imaging, metabolism measurements including NADH, FAD, tryptophan in cells and tissues and various scientific and clinical applications. On a personal note, the conference chairs are grateful for the participation of all authors, session chairs and acknowledge the innovation-driven manufacturers and sponsors of this conference [Applied Scientific Instruments (ASI), Becker & Hickl, Carl Zeiss. Chroma Technology, Coherent, ISS Inc., Excelitas Technologies, JenLab, Leica Microsystems, PicoQuant, Semrock (IDEX), and Spectra Physics-a division of MKS Instruments] for their enthusiastic support in organizing this conference successfully for the last 19 years. We look forward to other exciting conferences in the future and welcome your continued participation and support. Ammasi Periasamy Peter T. C. So Karsten König |
