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4 March 2019 Skin research with multiphoton FLIM (Conference Presentation)
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Multiphoton fluorescence lifetime imaging (FLIM) is gaining ground as a non-invasive and very sensitive research tool, and even as a method in clinical applications. Skin science is the predestined field for the latter, since skin is optically accessible without surgery. A hot topic is using metabolic imaging to investigate melanoma lesions. This method utilizes imaging of the ratio of the amounts of the free and protein-bound forms of the intracellular autofluorescent metabolic co-enzyme nicotinamide adenine dinucleotide (NADH) [1,2,3,4]. Another important topic which is closely bound up with skin cancer risk is safety aspects of sun screens. Multiphoton FLIM enables tracing of nanoparticle after application on the skin. Furthermore, in case of penetration through the stratum corneum again metabolic imaging can be used to investigate toxicity on skin cells [5]. References 1. O. Warburg, On the origin of cancer cells. Science (1956) 123:309-14 2. L. Pires, M.S. Nogueira, S. Pratavieira, et al. Time-resolved fluorescence lifetime for cutaneous melanoma detection. Biomed Opt Express (2014) 5:3080-9. 3. S. Seidenari, F. Arginelli, M. Manfredini, Multiphoton Laser Microscopy with Fluorescence Lifetime Imaging and Skin Cancer. In: Skin Cancer (Baldi A, Pasquali P, Spugnini EP, eds): (2014) Springer New York, 279-90. 4. M.N. Pastore, H. Studier H, C.S. Bonder, and M.S. Roberts. Non‐invasive metabolic imaging of melanoma progression. Exp Dermatol. (2016) 26:607–614. 5. A. M. Holmes, J. Lim, H. Studier, and M.S. Roberts, Varying the morphology of silver nanoparticles results in differential toxicity against micro-organisms, HaCaT keratinocytes and affects skin deposition. Nanotoxicology (2016) 10:10, 1503-1514
Conference Presentation
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Hauke Studier, Wolfgang Becker, Michael Roberts, Michael Pastore, and Amy Holmes "Skin research with multiphoton FLIM (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820L (4 March 2019);

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