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4 March 2019 Recent advances in femtosecond lasers for non-linear functional imaging of the brain and other tissues (Conference Presentation)
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Abstract
Functional non-linear imaging has become an essential tool to improve our understanding of how the brain works. Progress in neuroscience tools like functional probes and opsins, as well as novel imaging approaches using SLMs or adaptive optics enables to study hundreds or thousands of neurons at speeds matching the typical brain’s activity patterns. Studies in immunology and disease states on the other hand use more conventional lasers parameters and imaging tools, and benefit from a deeper integration of the laser with the microscope. In this presentation we will describe new generation lasers specifically designed to address the most advanced imaging needs in these two all-important areas. We will describe novel sources for functional brain imaging and optogenetics, based on Ytterbium fiber laser media that produce tens of watts of average power, energy per pulse of tens of microjoule at 1035 nm and unparalleled flexibility in repetition rate. Such high average powers/energies are necessary to stimulate large neuron populations when used for time interval shorter than the onset of cell damage; they can also be used to pump one or more wavelength conversion devices like OPAs and OPCPA used for 3-photon imaging and for fast volumetric Ca imaging. Application to stem cell research or disease states are generally less demanding in power and energy but requirements for high throughput and high quality images requires lasers tools that are more deeply integrated with the microscope providing fast dispersion and power management. In this presentation we will describe the state of the art of these different types of laser sources.
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Marco Arrigoni and Darryl McCoy "Recent advances in femtosecond lasers for non-linear functional imaging of the brain and other tissues (Conference Presentation)", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820R (4 March 2019); https://doi.org/10.1117/12.2510185
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