920-nm fiber laser delivering 100-fs pulses for nonlinear microscopy

Thomas Hellerer; Christoph Polzer; Axel Friedenauer; Isabel Greisberger; Bernhard Wolfring; Christoph Skrobol

doi:10.1117/12.2507753

22 February 2019 920-nm fiber laser delivering 100-fs pulses for nonlinear microscopy

Thomas Hellerer, Christoph Polzer, Axel Friedenauer, Isabel Greisberger, Bernhard Wolfring, Christoph Skrobol

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108820U (2019) https://doi.org/10.1117/12.2507753
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

This presentation focuses on ultrafast fiber lasers as excitation light-source for advanced microscopy. We will present our latest innovations and show how multiphoton microscopy can benefit from these developments. This will propel imaging techniques like TPEF, SHG, CARS or STED among others that excel conventional microscopy techniques in a deeper penetration depth, label-free imaging capability or higher spatial resolution. Wavelengths around 900 nm for addressing certain applications with fluorescent proteins and pulse durations as short as 100 fs remain a challenge for fiber lasers. TOPTICA has now launched the third generation of ultrafast fiber lasers overcoming these difficulties. The novel FF ultra laser platform is capable of generating such short pulses at not only the wavelengths 780 nm and 1050 nm but most recently at 920 nm too. This enables the imaging of the popular green fluorescent protein (GFP) and its derivatives with lowest possible power level because of the high efficiency. In the course of this work, we present selected applications proving the suitability of this industrial grade laser family for a broad variety of microscopic applications.

Conference Presentation

Citation Download Citation

Thomas Hellerer, Christoph Polzer, Axel Friedenauer, Isabel Greisberger, Bernhard Wolfring, and Christoph Skrobol "920-nm fiber laser delivering 100-fs pulses for nonlinear microscopy", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820U (22 February 2019); https://doi.org/10.1117/12.2507753

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available