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22 February 2019 920-nm fiber laser delivering 100-fs pulses for nonlinear microscopy
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Abstract
This presentation focuses on ultrafast fiber lasers as excitation light-source for advanced microscopy. We will present our latest innovations and show how multiphoton microscopy can benefit from these developments. This will propel imaging techniques like TPEF, SHG, CARS or STED among others that excel conventional microscopy techniques in a deeper penetration depth, label-free imaging capability or higher spatial resolution. Wavelengths around 900 nm for addressing certain applications with fluorescent proteins and pulse durations as short as 100 fs remain a challenge for fiber lasers. TOPTICA has now launched the third generation of ultrafast fiber lasers overcoming these difficulties. The novel FF ultra laser platform is capable of generating such short pulses at not only the wavelengths 780 nm and 1050 nm but most recently at 920 nm too. This enables the imaging of the popular green fluorescent protein (GFP) and its derivatives with lowest possible power level because of the high efficiency. In the course of this work, we present selected applications proving the suitability of this industrial grade laser family for a broad variety of microscopic applications.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas Hellerer, Christoph Polzer, Axel Friedenauer, Isabel Greisberger, Bernhard Wolfring, and Christoph Skrobol "920-nm fiber laser delivering 100-fs pulses for nonlinear microscopy", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108820U (22 February 2019); https://doi.org/10.1117/12.2507753
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