Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán; Philip Wijesinghe; Mingzhou Chen; Kishan Dholakia

doi:10.1117/12.2508373

22 February 2019 Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán, Philip Wijesinghe, Mingzhou Chen, Kishan Dholakia

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108821G (2019) https://doi.org/10.1117/12.2508373
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optical approaches have broadened their impact in recent years with innovations in both wide-field and super- resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multi- photon images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while significantly reducing photobleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

Conference Presentation

Citation Download Citation

Adrià Escobet-Montalbán, Philip Wijesinghe, Mingzhou Chen, and Kishan Dholakia "Wide-field multiphoton imaging with TRAFIX", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108821G (22 February 2019); https://doi.org/10.1117/12.2508373

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