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22 February 2019 Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser
Lin Huang, Shuo Tang
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Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108822G (2019) https://doi.org/10.1117/12.2505845
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signals simultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdoped fiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at 790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe. Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsic fluorophores, collagen, lipid and interface.
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Lin Huang and Shuo Tang "Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822G (22 February 2019); https://doi.org/10.1117/12.2505845
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KEYWORDS
Second-harmonic generation
Fiber lasers
Tissues
Endoscopy
Multiphoton microscopy
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