Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy

Álvaro Barroso Peña; Steffi Ketelhut; Peter Heiduschka; Geburg Nettels-Hackert; Jürgen Schnekenburger; Björn Kemper

doi:10.1117/12.2508976

21 February 2019 Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy

Álvaro Barroso Peña, Steffi Ketelhut, Peter Heiduschka, Geburg Nettels-Hackert, Jürgen Schnekenburger, Björn Kemper

Proceedings Volume 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI; 108830X (2019) https://doi.org/10.1117/12.2508976
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The refractive index (RI) of the retina and its dispersion are essential parameters in ophthalmologic imaging. However, the spatial RI distribution in retinal tissue is difficult to access. We explored the capabilities of multispectral quantitative phase imaging (QPI) with digital holographic microscopy (DHM) for label-free refractive index characterization of dissected murine retina. The retrieved tissue refractive indices are in agreement with previously reported values for living cells and dissected tissues. Moreover, the detected spatial refractive index distributions correlate with results from complementary conducted OCT investigations. In summary, multispectral DHM is a promising tool for label-free characterization of optical retina properties.

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Álvaro Barroso Peña, Steffi Ketelhut, Peter Heiduschka, Geburg Nettels-Hackert, Jürgen Schnekenburger, and Björn Kemper "Refractive index properties of the retina accessed by multi-wavelength digital holographic microscopy", Proc. SPIE 10883, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, 108830X (21 February 2019); https://doi.org/10.1117/12.2508976

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