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22 February 2019 Superresolution fluorescence microscopy using saturated modulation quenching (SMoQ)
Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar, Thomas Berer
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Proceedings Volume 10884, Single Molecule Spectroscopy and Superresolution Imaging XII; 108840U (2019) https://doi.org/10.1117/12.2510189
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
In this work, we demonstrate a new technique which has the potential for super-resolution fluorescence imaging. In this technique, similar to STED microscopy, a tightly focused intensity-modulated excitation beam and a donut shaped cw beam are confocally raster-scanned over the sample. In contrast to STED microscopy, both beams need to be absorbed by the fluorophore. A lock-in amplifier is used to measure only the modulated fluorescence. Sufficiently high cw donut beam intensities lead to saturation of the fluorophores in the outer rim of the modulated point spread function which enables resolution enhancement. Theoretically, sub-diffraction resolution can be achieved.
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Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Thomas A. Klar, and Thomas Berer "Superresolution fluorescence microscopy using saturated modulation quenching (SMoQ)", Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840U (22 February 2019); https://doi.org/10.1117/12.2510189
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KEYWORDS
Luminescence
Modulation
Microscopy
Quantum dots
Point spread functions
Resolution enhancement technologies
Super resolution
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