Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement

Dug Young Kim; Wonsang Hwang; Dong Eun Kim; Youngjae Won; Sucbei Moon; Sang Yoon Lee; Min Gu Kang; Won Sub Han

doi:10.1117/12.2510937

22 February 2019 Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement

Dug Young Kim, Wonsang Hwang, Dong Eun Kim, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Min Gu Kang, Won Sub Han

Author Affiliations +

Proceedings Volume 10884, Single Molecule Spectroscopy and Superresolution Imaging XII; 108840D (2019) https://doi.org/10.1117/12.2510937
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.

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Dug Young Kim, Wonsang Hwang, Dong Eun Kim, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Min Gu Kang, and Won Sub Han "Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement", Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840D (22 February 2019); https://doi.org/10.1117/12.2510937

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