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22 February 2019 Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement
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Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool widely used in monitoring cells, organelles, and tissues in biosciences. Since fluorescence lifetimes of most probes are a few nanoseconds, 20 ps measurement resolution is normally required. This requirement is quite challenging even with the fastest available optical and electronic devices, and several brilliant time-domain super-resolution techniques have been proposed for FLIM. The analog mean-delay (AMD) method is a recently introduced time-domain super-resolution technique for FLIM. Detailed constraints in the AMD method and their impact on the performance of the AMD super-resolution lifetime measurement are presented with experiments and simulations.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Dug Young Kim, Wonsang Hwang, Dong Eun Kim, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Min Gu Kang, and Won Sub Han "Analog mean-delay method: a new time-domain super-resolution technique for accurate fluorescence lifetime measurement", Proc. SPIE 10884, Single Molecule Spectroscopy and Superresolution Imaging XII, 108840D (22 February 2019); https://doi.org/10.1117/12.2510937
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