Imaging neural activity in zebrafish larvae with adaptive optics and structured illumination light sheet microscopy

Yang Liu; Keelan Lawrence; Aqsa Malik; Chelsea E. Gunderson; Rebecca Ball; James D. Lauderdale; Peter Kner

doi:10.1117/12.2507048

20 February 2019 Imaging neural activity in zebrafish larvae with adaptive optics and structured illumination light sheet microscopy

Yang Liu, Keelan Lawrence, Aqsa Malik, Chelsea E. Gunderson, Rebecca Ball, James D. Lauderdale, Peter Kner

Author Affiliations +

Proceedings Volume 10886, Adaptive Optics and Wavefront Control for Biological Systems V; 1088607 (2019) https://doi.org/10.1117/12.2507048
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Zebrafish are an important vertebrate model used to view the mechanisms underlying seizure disorders. Due to their relatively small size and transparency, larval zebrafish are an excellent model through which to view the occurence of seizure-like neural activity in vivo using light sheet fluorescence microscopy (LSFM). Although LSFM possesses good optical sectioning capability and high speed, the resolution and contrast degrade as the imaging plane is moved deeper into the sample due to refractive index variations. We have developed a system that combines a structured illumination light sheet microscope with adaptive optics in the emission path to correct optical aberrations and increase the resolution when imaging deep into the sample. We show that our system can record neural activity fast enough to capture seizure events, and is able to correct optical aberrations throughout the sample.

Citation Download Citation

Yang Liu, Keelan Lawrence, Aqsa Malik, Chelsea E. Gunderson, Rebecca Ball, James D. Lauderdale, and Peter Kner "Imaging neural activity in zebrafish larvae with adaptive optics and structured illumination light sheet microscopy", Proc. SPIE 10886, Adaptive Optics and Wavefront Control for Biological Systems V, 1088607 (20 February 2019); https://doi.org/10.1117/12.2507048

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