Quantitative phase microscopy for label-free evaluation of intestinal inflammation

Björn Kemper; Arne Bokemeyer; Steffi Ketelhut; Philipp Lenz; Dominik Bettenworth

doi:10.1117/12.2511947

4 March 2019 Quantitative phase microscopy for label-free evaluation of intestinal inflammation

Björn Kemper, Arne Bokemeyer, Steffi Ketelhut, Philipp Lenz, Dominik Bettenworth

Proceedings Volume 10887, Quantitative Phase Imaging V; 1088710 (2019) https://doi.org/10.1117/12.2511947
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

In overview, for the example of digital holographic microscopy (DHM), we present quantitative phase imaging (QPI)- based characterization of inflammation in colonic tissue sections. Tissue density alterations are determined by the refractive index retrieval from quantitative DHM phase images. We show that the average tissue refractive index represents a marker that allows to distinguish between different layers of the intestinal wall and that the revealed tissue refractive index data correlated well with the severity of inflammation. In summary, QPI provides biophysical parameters for the quantification of inflammation in intestinal tissues and paves the way for future applications in terms of ‘‘digital pathology’’.

Citation Download Citation

Björn Kemper, Arne Bokemeyer, Steffi Ketelhut, Philipp Lenz, and Dominik Bettenworth "Quantitative phase microscopy for label-free evaluation of intestinal inflammation", Proc. SPIE 10887, Quantitative Phase Imaging V, 1088710 (4 March 2019); https://doi.org/10.1117/12.2511947

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