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4 March 2019 Studying nucleic membrane fluctuations of tumor cells treated with chemotherapeutic drug using confocal reflectance quantitative phase microscopy (Conference Presentation)
Vijay Raj Singh, Zahid Yaqoob, Peter T. C. So
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Proceedings Volume 10887, Quantitative Phase Imaging V; 108871G (2019) https://doi.org/10.1117/12.2513575
Event: SPIE BiOS, 2019, San Francisco, California, United States
Abstract
Confocal reflectance quantitative phase microscopy system is developed in our lab to quantify nuclear membrane fluctuations. This system able to provide the µm level depth resolved phase information of the back scattered signal from nucleic membrane at ms temporal resolution. The phase information quantify the height fluctuations of nucleic membranes, which are subject to thermal fluctuations around the stable equilibrium in viscoelastic mediums. We further combined this system with Brillouin spectroscopic system, which measures longitudinal modulus of the nuclear material in the gigahertz (GHz) frequency range. Combining the information from confocal phase and Brillouin spectroscopy provides the nuclear membrane and material mechanical properties. Studies of anti-cancer drug effect on nuclear stiffness is performed on human lung cancer cells. Chemotherapeutic agent Doxorubicin (Dox) were used to treat these cancer cells and mechanical properties of nucleus were studied using the combined confocal and Brillouin spectroscopic system, as discussed above. The combined study of membrane fluctuations and stiffness measurements represent the positive correlation and indicate the softening the nuclei of tumor cells after treating with chemotherapeutic drug.
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Vijay Raj Singh, Zahid Yaqoob, and Peter T. C. So "Studying nucleic membrane fluctuations of tumor cells treated with chemotherapeutic drug using confocal reflectance quantitative phase microscopy (Conference Presentation)", Proc. SPIE 10887, Quantitative Phase Imaging V, 108871G (4 March 2019); https://doi.org/10.1117/12.2513575
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KEYWORDS
Confocal microscopy
Microscopy
Reflectivity
Tumors
Spectroscopy
Cancer
Lung cancer
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