Realization of isotropic resolution in optical diffraction tomography by exploiting a tomographic mould for optical trapping

Jeonghun Oh; Moosung Lee; Seungwoo Shin; YongKeun Park

doi:10.1117/12.2513335

25 March 2019 Realization of isotropic resolution in optical diffraction tomography by exploiting a tomographic mould for optical trapping

Jeonghun Oh, Moosung Lee, Seungwoo Shin, YongKeun Park

Author Affiliations +

Proceedings Volume 10887, Quantitative Phase Imaging V; 108872J (2019) https://doi.org/10.1117/12.2513335
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Optical diffraction tomography (ODT), which is one of the 3-D imaging techniques, enables non-invasive, label-free, and quantitative imaging of microscopic particles by retrieving multiple optical fields and reconstructing the 3-D hologram image of a sample. However, ODT employing the illumination scanning method suffers from poor axial resolution. In order to remedy this problem, we introduce TOmographic MOuld for optical TRAPping (TOMOTRAP) in ODT, which can control the orientation and shape of arbitrarily shaped dielectric objects. From optical field images of a sample rotated by several angles via TOMOTRAP, we reconstruct the 3-D RI distributions of samples with isotropic spatial resolution.

Citation Download Citation

Jeonghun Oh, Moosung Lee, Seungwoo Shin, and YongKeun Park "Realization of isotropic resolution in optical diffraction tomography by exploiting a tomographic mould for optical trapping", Proc. SPIE 10887, Quantitative Phase Imaging V, 108872J (25 March 2019); https://doi.org/10.1117/12.2513335

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