Raman tomography with frequency-modulated excitation and spatially-coded detection

Xixin Luo; Duofang Chen; Yonghua Zhan; Jimin Liang; Xueli Chen

doi:10.1117/12.2508455

4 March 2019 Raman tomography with frequency-modulated excitation and spatially-coded detection

Xixin Luo, Duofang Chen, Yonghua Zhan, Jimin Liang, Xueli Chen

Proceedings Volume 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019; 1089009 (2019) https://doi.org/10.1117/12.2508455
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Raman tomography can provide quantitative distribution of chemicals in a three-dimensional volume with a non-invasive and label-free manner. In view of the problems of existing data collection strategy, a frequency modulation and spatial encoding based Raman tomography was proposed, which aims to improve the data collection scheme and reduce the data collection time. In this scheme, the laser beam was divided into several sub-beams to use as multipoint excitation light sources. These sub-beams were first modulated with different frequencies and then incident on the different points of sample surface simultaneously. Because the excited Raman signals would carry such modulation information, the Raman signals from which excitation position can be distinguished with the demodulation process. In detection end, the Raman scattering light first passed through a spatial-encoding mask and then was directed to the single photomultiplier tube. By changing the pattern of the mask and then performing recovery with sparse reconstruction, the distribution of the Raman signals on the sample surface can be obtained based on compressive sensing theory. Preliminary results showed that our scheme can recover the Raman images to the certain extent with a better signal-to-noise ratio, demonstrating the proposed scheme is feasible.

Conference Presentation

Citation Download Citation

Xixin Luo, Duofang Chen, Yonghua Zhan, Jimin Liang, and Xueli Chen "Raman tomography with frequency-modulated excitation and spatially-coded detection", Proc. SPIE 10890, Label-free Biomedical Imaging and Sensing (LBIS) 2019, 1089009 (4 March 2019); https://doi.org/10.1117/12.2508455

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available